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Dr. Lee received her M.D. degree from University of California
at Davis in 1992. She trained in internal medicine at the Harbor-UCLA
Medical Center and subsequently did her fellowship in pulmonary
and critical medicine at the University of California at San Francisco.
She is a member of the Lung Biology Center, where she investigates
cytokine mediated gene expression in resident airway cells. She
joined the UCSF division of pulmonary and critical care medicine
in 2001 and attends on the pulmonary consult service and in the
intensive care unit. She is also the director of the High Risk
Asthma Clinic at San Francisco General Hospital.
Resident airway cells are important effector cells in the pathogenesis
of airway diseases such as asthma. Study of gene expression profile
of several airway cell types in response to IL-13 has revealed
that each cell type has a distinct transcriptional response to
the same stimuli despite activation of the same signaling pathway.
Furthermore, among the genes with significant change in expression
level in response to the stimuli, little to no overlap exists
between the cell types. From these results, it is clear that each
airway cell type has very unique contributions to disease pathogenesis.
My research involves comprehensive gene expression profiling of
resident airway cells using oligonucleotide arrays. In particular,
I am focusing on transcriptional similarities and differences
in normal human bronchial epithelial cells (NHBE), bronchial smooth
muscle cells (BSMC), and normal human lung fibroblasts (NHLF)
in response to cytokines relevant to airway diseases such as asthma.
This approach of unbiased gene expression analysis allows identification
of candidate genes and the airway cell type that may contribute
to the disease pathogenesis. We’ve already identified a number
of genes which may be of biological importance in their contributions
to important pathophysiologic features of asthma, airway hyperresponsiveness
and airway remodeling. These candidates are being examined both
in vitro and in vivo for functional significance. Ultimately,
the identification of specific genes and cell types will lead
to design of better therapeutic interventions for the treatment
of lung diseases.
Lee JH, Kaminski N, Dolganov G, Gruenig G, Koth L, Solomon C,
Sheppard D. Interleukin-13 induces dramatically different transcriptional
programs in three human airway cell types. Am. J. Respir. Cell
Mol. Biol. 2001 25: 474-485 (abstract)/(full
text)
Huang XZ, Wu JF, Ferrando R, Lee JH, Wang YL, Farese RV Jr., Sheppard
D. Fatal Bilateral Chylothorax in Mice Lacking the Integrin a9b1.
2000. Mol Cell Bio, 20(14): 5208-5215. (abstract)/(full
text)
Lee JH, Kaminski N, Gruenig G, Sheppard D. IL-13 Induced Gene
Expression in Bronchial Smooth Muscle Cells. 2000. Am J Respir
Crit Care Med, 161(3): A699
Lee JH, Huang XZ, Sheppard D. IL-11 Induction of Integrin avb6
Expression on Human Bronchial Epithelial Cells is Mediated by
TGFb. 2000. Am J Respir Crit Care Med, 161(3): A248
Kaminski N, Lee JH, Allard J, Heller RA, Sheppard D. TGFb Induces
Distinct Transcriptional Programs in Airway Epithelial and Airway
Smooth Muscle Cells. 2000. Am J Respir Crit Care Med, 161(3):
A667
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