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Dr. Woodruff received his B.A, from Wesleyan University in 1989, received his M.D. degree from the Columbia College of Physicians and Surgeons in 1993, and completed Internal Medicine residency training at the Massachusetts General Hospital. After residency training, he obtained research training as a Research Fellow in the Channing Laboratory, Division of Respiratory Epidemiology, at the Brigham and Women’s Hospital, and pursued graduate studies leading to a Masters in Public Health from the Harvard School of Public Health in 1998. He undertook fellowship training in Pulmonary and Critical Care Medicine here at UCSF beginning in 1998 and joined the UCSF faculty in 2002. He is currently an Associate Professor of Medicine in the Department of Medicine and the Cardiovascular Research Institute and is Associate Director of the UCSF Airway Clinical Research Center.
I. The identification of molecular sub-phenotypes of asthma and COPD:
These studies are funded by:
1) An R01 from the NIH/NHLBI, entitled “Molecular Phenotyping of Asthma” (R01 HL-095372) which has applied genomic analyses to airway samples from patients with asthma to distinguish Th2-driven and non-Th2-driven sub-phenotypes of asthma that have distinct clinical, pathological and treatment-related characteristics.
2) A seven-year NIH contract to apply similar methods to study COPD as part of the NHLBI Spiromics Project (N01 HR-08-08). The goal of the Spiromics project is to identify molecular phenotypes of COPD and to develop intermediate outcome measures for clinical trials.
II. Mechanisms of airway inflammation and remodeling in lung diseases:
These studies apply innovative methods for quantitative morphometry and gene expression analyses to human tissue samples obtained at fiberoptic bronchoscopy. In work to date, we have applied these methods to study mechanisms of disease in asthma, COPD and sarcoidosis. Ongoing work in this area is supported by the NIH (R01 HL-095372). Our approaches combine the application of gene expression profiling methods (microarrays, qPCR, laser capture microdissection) and design–based stereology (for quantitative measurement of tissue remodeling in human samples).
III. Development of blood-based diagnostic tests for lung disease using genomic approaches
These studies apply gene expression profiling and miRNA profiling in blood cells and plaema/serum to develop diagnostic tests in asthma, COPD and sarcoidosis.
IV. Sample analysis and support for Clinical Trials:
Finally, the Woodruff Laboratory supports sample analyses for clinical trials in asthma and COPD in both NIH and industry-supported studies. These sample analyses include measurement of changes in airway remodeling in response to specific therapeutic interventions, assessment of inflammation and the application of biomarkers to enhance the interpretation of clinical trials.
Albert RK, Connett J, Bailey WC, Casaburi R, Cooper JAD, Criner GJ, Curtis JL, Dransfield MT, Han MK, Lazarus SC, Make B, Marchetti N, Martinez FJ, Madinger NA, McEvoyC, Niewoehner DE, Porsasz J, Price, CS, Reilly J, Scanlon PD, Sciurba FC, Scharf SM, Washko GR, Woodruff PG, and Anthonisen NR. for the COPD Clinical Research Network. Azithromycin for Prevention of Exacerbations of COPD. N Engl J Med 2011; 365:689-698
Koth LL, Solberg OD, Peng JC, Bhakta NR, Nguyen CP, Woodruff PG. Sarcoidosis Blood Transcriptome Reflects Lung Inflammation and Overlaps with Tuberculosis. Am J Respir Crit Care Med. 2011 Aug 18. [Epub ahead of print]
Choy DF, Modrek B, Abbas AR, Kummerfeld S, Clark HF, Wu LC, Fedorowicz G, Modrusan Z, Fahy JV, Woodruff PG, Arron JR. Gene expression patterns of th2 inflammation and intercellular communication in asthmatic airways. J Immunol. 2011 Feb 1;186(3):1861-9. PMC Journal – In Process
Woodruff PG, Albert RK, Bailey WC, Casaburi R, Connett JE, Cooper JAD, Criner GJ, Curtis JL, Dransfield MT, Han MK, Harnden SM, Kim V, Marchetti N, Martinez FJ, McEvoy CE, Niewoehner DE, Reilly JJ, Rice K, Scanlon PD, Scharf SM, Sciurba FC, Washko GR, Lazarus SC for the COPD Clinical Research Network. Randomized Trial of Zileuton for Treatment of COPD Exacerbations Requiring Hospitalization. COPD. 2011 Feb;8(1):21-9. PMC Journal – In Process
Bhakta N, Woodruff PG. Human Asthma Phenotypes: From the Clinic, to Cytokines, and Back Again Immunol Rev. 2011 Jul;242(1):220-32.
Woodruff PG. Novel Outcomes and End-points: Biomarkers in COPD Clinical Trials. Proc Am Thorac Soc. 2011 Aug;8(4):350-5.
Sidhu SS, Yuan S, Innes AL, Woodruff PG, Solon M, Hou L, Muller SJ, and Fahy JV.Epithelial cell-derived periostin: roles in TGFbeta activation, collagen production and collagen gel elasticity in asthma. Proc Natl Acad Sci U S A. 2010 Aug 10;107(32):14170-5. PMCID: PMC2922596
Innes AL, McGrath KW, Dougherty RH, McCulloch CE, Woodruff PG, Seibold MA, Okamoto KS, Ingmundson KJ, Solon M, Carrington SD, Fahy JV. The H Antigen at Epithelial Surfaces is Associated with Susceptibility to Asthma Exacerbation. Am J Respir Crit Care Med. 2011 Jan 15;183(2):189-94. PMC Journal – In Process
Koth LL, Cambier CJ, Ellwanger A, Solon M, Hou L, Lanier LL, Abram CL, Hamerman JA, Woodruff PG. DAP12 is required for macrophage recruitment to the lung in response to cigarette smoke and chemotaxis toward CCL2. J Immunol. 2010 Jun 1;184(11):6522-8. Epub 2010 Apr 26. PubMed PMID: 20421649.
Dougherty RH, Sidhu SS, Raman K, Solon M, Solberg OD, Caughey GH, Woodruff PG, Fahy JV. Accumulation of intraepithelial mast cells with a unique protease phenotype in T(H)2-high asthma. J Allergy Clin Immunol. 2010 May;125(5):1046-1053.e8.
Woodruff PG, Wolff M, Hohlfeld JM, Krug N, Dransfield MT, Sutherland ER, Criner GJ, Kim V, Prasse A, Nivens MC, Tetzlaff K, Heilker R, Fahy JV. Safety and Efficacy of an Inhaled Epidermal Growth Factor Receptor Inhibitor (BIBW 2948 BS) in COPD. Am J Respir Crit Care Med. 2010 Mar 1;181(5):438-45.
Hsia CC, Hyde DM, Ochs M, Weibel ER; ATS/ERS Joint Task Force on Quantitative Assessment of Lung Structure. An official research policy statement of the American Thoracic Society/European Respiratory Society: standards for quantitative assessment of lung structure. Am J Respir Crit Care Med. 2010 Feb 15;181(4):394-418.
Park SW, Verhaeghe C, Nguyenvu LT, Barbeau R, Eisley CJ, Nakagami Y, Huang X, Woodruff PG, Fahy JV, Erle DJ. Roles of FOXA2 and FOXA3 in Allergic Airway Disease and Asthma. Am J Respir Crit Care Med. Jul 23 2009 Oct 1;180(7):603-10. PMCID: PMC2753788
Dransfield MT, Nahm MH, Han MK, Harnden S, Criner GJ, Martinez FJ, Scanlon PD, Woodruff PG, Washko GR, Connett JE, Anthonisen NR, Bailey WC. Superior Immune Response to Protein-conjugate vs. Free Pneumococcal Polysaccharide Vaccine in COPD. Am J Respir Crit Care Med. 2009 Sep 15;180(6):499-505. PMCID: PMC2742743
Woodruff PG, Modrek B, Choy DF, Jia G, Abbas AR, Ellwanger A, Koth LL, Arron JR, and Fahy JV. Th2-driven inflammation defines major sub-phenotypes of asthma. Am J Respir Crit Care Med 2009 Sep 1;180(5):388-95. PMCID: PMC2742757 |
